Journal: BMC Genomics

Article Title: Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation

doi: 10.1186/s12864-015-1694-y

Figure Lengend Snippet: Effect of stabilization reagent, storage time and storage temperature on RNA integrity. Experimental design ( a ) and RNA integrity ( b ) of total RNA extracted from stool samples conserved in four different stabilizing reagents for up to 15 days at room temperature and 4 °C

Article Snippet: We therefore compared RNA stability in 3 different RNA stabilizing reagents (Stool Total RNA Purification Kit (Norgen), Powermicrobiome RNA Isolation Kit (MoBio) and RNeasy Mini Kit (Qiagen)) at two different storage temperatures (4 °C and RT) for up to 360 h. In addition, the DNA stabilizer solution of the PSP Spin Stool DNA Plus Kit (Stratec, Germany) was also included since this kit would allow to simultaneously isolate RNA and DNA.

Techniques:

Journal: PLoS ONE

Article Title: Intralipid Decreases Apolipoprotein M Levels and Insulin Sensitivity in Rats

doi: 10.1371/journal.pone.0105681

Figure Lengend Snippet: Total RNA was obtained from the liver of rats after infusion of 5% glucose solution (controls, n = 6) or rats infused with 20% Intralipid solution (n = 6). mRNA expression was determined by real-time PCR. mRNA levels in control rats were set as 100%. Data are presented as means ± SEM.

Article Snippet: Total RNA from 293T cells, liver tissues from SD rats and liver, kidney and lung tissues from GK rats was extracted using a total RNA purification kit (Omega Bio-Tek Inc., Norcross, GA, USA), according to the manufacturer's instructions.

Techniques: Expressing, Real-time Polymerase Chain Reaction